《植物生理学报》 2013, 49(1): 47-52

番茄果实成熟过程中番茄红素含量及合成相关基因表达的分析

刘英明, 姜晶*, 王晶, 杨艳丽, 孟昭娟

沈阳农业大学园艺学院, 设施园艺省部共建教育部重点实验室, 辽宁省设施园艺重点实验室, 沈阳110866

通信作者：姜晶；E-mail: jiangjingcau@163.com；Tel: 024-88487143

摘　要：

以2个高代自交系粉果番茄MLK1和红果番茄FL1为材料, 利用实时荧光定量PCR技术及色差仪法, 对果实成熟过程中4个时期的番茄红素含量分析及八氢番茄红素合成酶(Psy1和Psy2)和番茄红素环化酶(Lcy)基因的表达进行研究。结果表明, 在番茄果实成熟的过程中, 番茄红素的含量也逐渐增高, 在完熟期达到最高, 且红果中的含量高于粉果中的。在2个番茄品种果实不同部位中, Psy1、Psy2和Lcy基因在果实逐渐成熟的过程中转录水平均逐渐增加, 在完熟期表达量最高, 且红果FL1中的表达量高于粉果MLK1表达量, 果实中Psy基因的表达量高于Lcy基因的表达量。

关键词：番茄; 番茄红素; 实时荧光定量PCR; 基因表达

收稿：2012-10-11   修定：2012-12-26

资助：国家自然科学基金项目(30971999)和辽宁省教育厅重点实验室项目课题(2009S052)。

Analysis of Lycopene Content and Biosynthetic Related Gene Expression during Maturation of Tomato Fruit

LIU Ying-Ming, JIANG Jing*, WANG Jing, YANG Yan-Li, MENG Zhao-Juan

Key Laboratory of Protected Horticulture, Ministry of Education, Key Laboratory of Protected Horticulture of Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: JIANG Jing; E-mail: jiangjingcau@163.com; Tel: 024-88487143

Abstract:

Two tomato cultivars red fruit FL1 and pink fruit MLK1 with significant difference in lycopene con-tent were used for the study of the lycopene content and the gene expression of phytoene synthase (Psy1 and Psy2) and lycopene cyclase (Lcy) in different organs and at four development stages of fruits. The results showed that the content of lycopene gradually increased in tomato fruit ripening process and was the highest in the ripe stage, the content of lycopene in red fruit FL₁ was higher than that in pink fruit MLK₁ tomato. Three genes were expressed in different parts of the two varieties tomato fruits in the transcription level and the ex-pression levels were the highest at the ripe stage. The genes expression of red fruit FL₁ was higher than those in pink fruit MLK₁. Psy gene expression level was higher than the expression levels of Lcy gene in the same vari-eties of fruit.

Key words: tomato; lycopene; real-time quantitative PCR; gene expression